Lipocalin 2 prevents intestinal inflammation by enhancing phagocytic bacterial clearance in macrophages

• 松浦, 稔

• 本澤, 有介

• 山田, 聡

• Honzawa, Yusuke

• Minami, Naoki

• Yamada, Satoshi

• Kobayashi, Taku

• Hibi, Toshifumi

• Nakase, Hiroshi

抄録

Lipocalin 2 (Lcn2), also called neutrophil gelatinase B-associated lipocalin (NGAL), is an anti-microbial peptide originally identified in neutrophil granules. Although Lcn2/NGAL expression is increased in the inflamed intestinal tissues of patients with inflammatory bowel disease, the role of Lcn2/NGAL in the development of intestinal inflammation remains unclear. Here we investigated the role of Lcn2/NGAL in intestinal inflammation using a spontaneous mouse colitis model, interleukin-10 knock out (IL-10 KO) mice. Lcn2 expression in the colonic tissues of IL-10 KO mice increased with the development of colitis. Lcn2/IL-10 double-KO mice showed a more rapid onset and development of colitis compared to IL-10 KO mice. Lcn2 enhanced phagocytic bacterial clearance in macrophages in vitro after infection with Escherichia coli. Transfer of Lcn2-repleted macrophages prevented the development of colitis in Lcn2/IL-10 double-KO mice in vivo. Our findings revealed that Lcn2 prevents the development of intestinal inflammation. One crucial factor seems to be the enhancement of phagocytic bacterial clearance in macrophages by Lcn2.

収録刊行物

• Scientific Reports

  Scientific Reports 6 2016-10-13

  Springer Nature

キーワード

• Inflammatory bowel disease
• Monocytes and macrophages

詳細情報

• CRID: 1050001335849270656
• NII論文ID: 120006318859
• ISSN: 20452322
• HANDLE: 2433/226284
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles