Dentin resorption and cementum-like tissue formation by bone morphogenetic protein application

• Miyaji, Hirofumi

• Sugaya, Tsutomu

• Kato, Katsuya

• Kawamura, Naoto

• Tsuji, Hifumi

• Kawanami, Masamitsu

書誌事項

タイトル別名

• Dentin resorption and cementum-like tissue formation by BMP application
• Tissue reactions on BMP-applied dentin

抄録

Objective: This study aimed to examine the effect of bone morphogenetic protein-2 (BMP-2) application on dentin resorption and cementum-like tissue formation at the dentin surfaces. Background: Recent studies showed that BMP-2 stimulated the mineralization and osteoclast differentiation. Osteoclastic resorption by BMP-2 application may play an important role in the regulation of new cementum-like tissue formation on the dentin surfaces. Methods: Seventy-two flat dentin blocks were prepared from rat roots, treated with 24 % EDTA, applied with 0, 100, and 400 μg/ml BMP-2, and labeled as groups 0, 100, and 400. The dentin blocks were then implanted into palatal connective tissue of rats, and specimens were prepared at two, four and eight weeks after surgery for histologic and histomorphometric analyses. Results: BMP-2 caused a dose-dependent increase in dentin resorption by osteoclastic cells. New cementum-like tissue was randomly formed on parts of the non-resorbed and resorbed dentin surfaces in groups 100 and 400. Dentin resorption in groups 100 and 400 was significantly greater than group 0 (p<0.01). However, at eight weeks new cementum-like tissue formed in 41.8% of group 100, as compared to 16.2% in group 400 (p<0.05). Conclusion: Dentin resorption was stimulated by a high dose of BMP-2, and cementum-like tissue was induced by a low dose of BMP-2, effectively suggesting that BMP-2 application in an appropriate dose to a dentin surface may enhance periodontal regeneration.

収録刊行物

• Journal of Periodontal Research

  Journal of Periodontal Research 41 (4), 311-315, 2006-08

  Wiley-Blackwell

キーワード

• BMP (bone morphogenetic protein)
• Dentin
• Resorption
• Cementum like tissue

詳細情報

• CRID: 1050001339023101824
• NII論文ID: 120006323697
• NII書誌ID: AA00704381
• ISSN: 16000765
• ISSN: 00223484
• HANDLE: 2115/66596
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles