p53 represses the transcription of snRNA genes by preventing the formation of little elongation complex

• Anwar, Delnur

• Takahashi, Hidehisa

• Watanabe, Masashi

• Suzuki, Masanobu

• Fukuda, Satoshi

• Hatakeyama, Shigetsugu

Abstract

The regulation of transcription by RNA polymerase II (Pol II) is important for a variety of cellular functions. ELL/EAF-containing little elongation complex (LEC) was found to be required for transcription of Pol II-dependent small nuclear RNA (snRNA) genes. It was shown that the tumor suppressor p53 interacts with ELL and inhibits transcription elongation activity of ELL Here, we show that p53 inhibits interaction between ELL/EAF and ICE1 in LEC and thereby p53 represses transcription of Pol II-dependent snRNA genes through inhibiting LEC function. Furthermore, induction of p53 expression by ultraviolet (UV) irradiation decreases the occupancy of ICE1 at Pol II-dependent snRNA genes. Consistent with the results, knockdown of p53 increased both the expression of snRNA genes and the occupancy of Pol II and components of LEC at snRNA genes. Our results indicate that p53 interferes with the interaction between ELL/EAF and ICE1 and represses transcription of snRNA genes by Pol II.

Journal

• Biochimica et biophysica acta. Gene regulatory mechanisms

  Biochimica et biophysica acta. Gene regulatory mechanisms 1859 (8), 975-982, 2016-08

  Elsevier

Keywords

• p53
• ELL
• Transcription
• snRNA
• LEC

Details

• CRID: 1050001339020657664
• NII Article ID: 120006328999
• HANDLE: 2115/66903
• ISSN: 18749399
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles