Sardine procalcitonin amino-terminal cleavage peptide has a different action from calcitonin and promotes osteoblastic activity in the scales of goldfish

• Kase Yoichi

• Ikari Takahiro

• Sekiguchi Toshio

• Sato Masayuki

• Ogiso Shouzo

• Kawada Tsuyoshi

• Matsubara Shin

• Satake Honoo

• Sasayama Yuichi

• Endo Masato

• Kitamura Kei-ichiro

• Hattori Atsuhiko

• Watanabe Takushi X.

• Maruyama Yusuke

• Watanabe Yoshinari

• Funahashi Hisayuki

• Kambegawa Akira

• Suzuki Nobuo

• 関口 俊男

• 佐藤 政行

• 笹山 雄一

• 北村 敬一郎

• 渡辺 良成

• 鈴木 信雄

Abstract

The nucleotide sequence of a sardine preprocalcitonin precursor has been determined from their ultimobranchial glands in the present study. From our analysis of this sequence, we found that sardine procalcitonin was composed of procalcitonin amino-terminal cleavage peptide (N-proCT) (53 amino acids), CT (32 amino acids), and procalcitonin carboxyl-terminal cleavage peptide (C-proCT) (18 amino acids). As compared with C-proCT, N-proCT has been highly conserved among teleosts, reptiles, and birds, which suggests that N-proCT has some bioactivities. Therefore, both sardine N-proCT and sardine CT were synthesized, and their bioactivities for osteoblasts and osteoclasts were examined using our assay system with goldfish scales that consisted of osteoblasts and osteoclasts. As a result, sardine N-proCT (10− 7 M) activated osteoblastic marker enzyme activity, while sardine CT did not change. On the other hand, sardine CT (10− 9 to 10− 7 M) suppressed osteoclastic marker enzyme activity, although sardine N-proCT did not influence enzyme activity. Furthermore, the mRNA expressions of osteoblastic markers such as type 1 collagen and osteocalcin were also promoted by sardine N-proCT (10− 7 M) treatment; however, sardine CT did not influence their expressions. The osteoblastic effects of N-proCT lack agreement. In the present study, we can evaluate exactly the action for osteoblasts because our scale assay system is very sensitive and it is a co-culture system for osteoblasts and osteoclasts with calcified bone matrix. Both CT and N-proCT seem to influence osteoblasts and osteoclasts and promote bone formation by different actions in teleosts. © 2017 Elsevier Inc.

Embargo Period 12 months

Journal

• Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology

  Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology 211 77-83, 2017-09-01

  Elsevier

Keywords

• Calcitonin
• Japanese sardine
• Osteoblasts
• Osteoclasts
• Procalcitonin amino-terminal cleavage peptide
• Scales

Details

• CRID: 1390577441952829056
• NII Article ID: 120006336026
• NII Book ID: AA11183804
• ISSN: 10956433
• DOI: 10.24517/00029380
• DOI: 10.1016/j.cbpa.2017.06.007
• Web Site: http://hdl.handle.net/2297/48459
• Web Site: https://api.elsevier.com/content/article/PII:S1095643317301356?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S1095643317301356?httpAccept=text/plain
• Text Lang: en
• Data Source:
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  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN