Arabidopsis R1R2R3-Myb proteins are essential for inhibiting cell division in response to DNA damage

• Chen, Poyu

• Takatsuka, Hirotomo

• Takahashi, Naoki

• Kurata, Rie

• Fukao, Yoichiro

• Kobayashi, Kosuke

• Ito, Masaki

• Umeda, Masaaki

抄録

Inhibition of cell division is an active response to DNA damage that enables cells to maintain genome integrity. However, how DNA damage arrests the plant cell cycle is largely unknown. Here, we show that the repressor-type R1R2R3-Myb transcription factors (Rep-MYBs), which suppress G2/M-specific genes, are required to inhibit cell division in response to DNA damage. Knockout mutants are resistant to agents that cause DNA double-strand breaks and replication stress. Cyclin-dependent kinases (CDKs) can phosphorylate Rep-MYBs in vitro and are involved in their proteasomal degradation. DNA damage reduces CDK activities and causes accumulation of Rep-MYBs and cytological changes consistent with cell cycle arrest. Our results suggest that CDK suppressors such as CDK inhibitors are not sufficient to arrest the cell cycle in response to DNA damage but that Rep-MYB-dependent repression of G2/M-specific genes is crucial, indicating an essential function for Rep-MYBs in the DNA damage response.

収録刊行物

• Nature Communications

  Nature Communications 8 (1), 635-, 2017-09-21

  Springer Nature

キーワード

• General Physics and Astronomy
• General Biochemistry, Genetics and Molecular Biology
• General Chemistry
• Multidisciplinary

詳細情報

• CRID: 1050014359399365376
• NII論文ID: 120006354238
• NII書誌ID: AA12645905
• ISSN: 20411723
• DOI: 10.1038/s41467-017-00676-4
• HANDLE: 10061/12136
• Web Site: https://naist.repo.nii.ac.jp/records/9180
• Web Site: https://www.nature.com/articles/s41467-017-00676-4.pdf
• Web Site: https://www.nature.com/articles/s41467-017-00676-4
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN