Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA

• Kubo, Minoru

• Nango, Eriko

• Tono, Kensuke

• Kimura, Tetsunari

• Owada, Shigeki

• Song, Changyong

• Mafune, Fumitaka

• Miyajima, Ken

• Takeda, Yoshihiro

• Kohno, Jun-ya

• Miyauchi, Naoya

• Nakane, Takanori

• Tanaka, Tomoyuki

• Nomura, Takashi

• Davidsson, Jan

• Tanaka, Rie

• Murata, Michio

• Kameshima, Takashi

• Hatsui, Takaki

• Joti, Yasumasa

• Neutze, Richard

• Yabashi, Makina

• Iwata, So

Abstract

X-ray free-electron lasers (XFELs) have opened new opportunities for timeresolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TRSFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented.

Journal

• Journal of Synchrotron Radiation

  Journal of Synchrotron Radiation 24 (5), 1086-1091, 2017-09-01

  International Union of Crystallography

Details

• CRID: 1050856995323810560
• NII Article ID: 120006363292
• ISSN: 16005775
• ISSN: 09090495
• HANDLE: 20.500.14094/90004340
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles