Dual role of the active-center cysteine in human peroxiredoxin 1 : Peroxidase activity and heme binding

• Watanabe, Yuta

• Ishimori, Koichiro

• Uchida, Takeshi

Abstract

HBP23, a 23-kDa heme-binding protein identified in rats, is a member of the peroxiredoxin (Prx) family, the primary peroxidases involved in hydrogen peroxide catabolism. Although HBP23 has a characteristic Cys-Pro heme-binding motif, the significance of heme binding to Prx family proteins remains to be elucidated. Here, we examined the effect of heme binding to human peroxiredoxin-1 (PRX1), which has 97% amino acid identity to HBP23. PRX1 was expressed in Escherichia coli and purified to homogeneity. Spectroscopic titration demonstrated that PRX1 binds heme with a 1:1 stoichiometry and a dissociation constant of 0.17 mu M. UV-vis spectra of heme-PRX1 suggested that Cys52 is the axial ligand of ferric heme. PRX1 peroxidase activity was lost upon heme binding, reflecting the fact that Cys52 is not only the heme-binding site but also the active center of peroxidase activity. Interestingly, heme binding to PRX1 caused a decrease in the toxicity and degradation of heme, significantly suppressing H2O2-dependent heme peroxidase activity and degradation of PRX1-bound heme compared with that of free hemin. By virtue of its cytosolic abundance (similar to 20 mu M), PRX1 thus functions as a scavenger of cytosolic hemin (<1 mu M). Collectively, our results indicate that PRX1 has a dual role; Cys-dependent peroxidase activity and cytosolic heme scavenger. (C) 2017 Elsevier Inc. All rights reserved.

Journal

• Biochemical and biophysical research communications

  Biochemical and biophysical research communications 483 (3), 930-935, 2017-02-12

  Elsevier

Keywords

• Heme
• Peroxiredoxin
• Cytosolic heme-binding protein

Details

• CRID: 1050282813999595392
• NII Article ID: 120006383811
• HANDLE: 2115/68306
• ISSN: 0006291X
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles