DIOL DERIVATIVE OF (3-TRIFLUOROMETHYL)PHENYLDIAZIRINE FOR POST-LABELING OF PHOTOCROSSLINK

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3-Trifluoromethylphenyldiazirinylated diol derivative was utilized to introduce an aldehyde by periodate oxidation, followed by the formation of a Schiff base with biotin hydrazide on a PVDF membrane for post-labeling of photocrosslinked proteins. The biotin hydrazide was able to post-label the 2.5 x 10-13 mole of closslinked component for chemiluminescent visualization.

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