タバコ懸濁培養細胞のオーキシン独立な細胞分裂を誘導する 新奇糖タンパク質(NtXYL1)の発現および機能解析

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For plant cell growth, it is generally necessary to supply factors that induce cell division such as auxin. However, it is known that cell proliferation becomes possible without the addition of auxin to the medium during the continuous cell culture. This phenomenon that physiologically adapts to changes in the environment is called habituation. In tobacco cell lines, that could proliferate without auxin was isolated in which a glycoprotein named NtXYL1 was thought to be involved. In this study, to investigate the involvement of NtXYL1 in the auxin-independent cell proliferation, gene expression of NtXYL1 was examined during cell proliferation and the NtXYL1-overexpressing cell lines were prepared. As I found that several overexpressing lines could grow on the medium with a reduced auxin concentration, NtXYL1 was considered to be involved in the auxin-independent cell proliferation. Although gene expression of NtXYL1 was auxin-independent, the relationship between the induction of cell division by auxin and the function of NtXYL1 was discussed. Key Words: auxin , cell division, glycoprotein, NtXYL1

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