Involvement of the nuclear progestin receptor in LH-induced expression of membrane type 2-matrix metalloproteinase required for follicle rupture during ovulation in the medaka, Oryzias latipes

• Ogiwara, Katsueki

• Takahashi, Takayuki

抄録

Hormonal regulation of the expression of Mmp15, a proteolytic enzyme indispensable for ovulation in the teleost medaka, was investigated. In an in vitro culture system using preovulatory follicles, Mmp15 expression and ovulation were induced in the presence of recombinant luteinizing hormone (rLh). Both rLh-induced Mmp15 expression and ovulation were 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one-dependent, suggesting the involvement of a nuclear progestin receptor (Pgr). In vitro follicle ovulation and Mmp15 expression were reduced by treatment with the Pgr antagonist RU-486. Like Pgr, the transcription factor CCAAT/enhancer-binding protein beta (Cebpb) was induced by rLh. ChIP analyses indicated that Pgr and Cebpb bound to the mmpl5 promoter region. These results indicate that the rLh-induced expression of Mmp15 is mediated by Pgr and Cebpb. A differential timing of expression of Pgr and Cebpb in the preovulatory follicles appears to explain the considerably long time-lag from the pgr gene activation to mmpl5 gene expression. (C) 2017 Elsevier B.V. All rights reserved.

収録刊行物

• Molecular and cellular endocrinology

  Molecular and cellular endocrinology 450 54-63, 2017-07-15

  Elsevier

キーワード

• Medaka
• Ovulation
• LH
• Mmp15
• Nuclear progestin receptor

詳細情報

• CRID: 1050845763953223680
• NII論文ID: 120006454483
• HANDLE: 2115/68833
• ISSN: 03037207
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles