A spliced latency-associated VZV transcript maps antisense to the viral transactivator gene 61

• Depledge, Daniel P.

• Ouwendijk, Werner J. D.

• 定岡, 知彦

• Braspenning, Shirley E.

• 森, 康子

• Cohrs, Randall J.

• Verjans, Georges M. G. M.

• Breuer, Judith

抄録

Varicella-zoster virus (VZV), an alphaherpesvirus, establishes lifelong latent infection in the neurons of > 90% humans worldwide, reactivating in one-third to cause shingles, debilitating pain and stroke. How VZV maintains latency remains unclear. Here, using ultra-deep virus-enriched RNA sequencing of latently infected human trigeminal ganglia (TG), we demonstrate the consistent expression of a spliced VZV mRNA, antisense to VZV open reading frame 61 (ORF61). The spliced VZV latency-associated transcript (VLT) is expressed in human TG neurons and encodes a protein with late kinetics in productively infected cells in vitro and in shingles skin lesions. Whereas multiple alternatively spliced VLT isoforms (VLTly) are expressed during lytic infection, a single unique VLT isoform, which specifically suppresses ORF61 gene expression in co-transfected cells, predominates in latently VZV-infected human TG. The discovery of VLT links VZV with the other better characterized human and animal neurotropic alphaherpesviruses and provides insights into VZV latency.

収録刊行物

• Nature Communications

  Nature Communications 9 1167-1167, 2018-03-21

  Nature Publishing Group

詳細情報

• CRID: 1050575520346895744
• NII論文ID: 120006454532
• DOI: 10.1038/s41467-018-03569-2
• ISSN: 20411723
• HANDLE: 20.500.14094/90004753
• Web Site: https://www.nature.com/articles/s41467-018-03569-2.pdf
• Web Site: https://www.nature.com/articles/s41467-018-03569-2
• 本文言語コード: en
• 資料種別: journal article
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