HPLC-UVを用いたソホスブビルの定量法の確立 Determination of sofosbuvir via a high-performance liquid chromatography method using ultraviolet detection

この論文にアクセスする

抄録

Sofosbuvir (SOF) is a nucleotide analog drug that inhibits hepatitis C virus replication. In the present study, we aimed todevelop a simple, specific, sensitive, and precise method for the quantification of SOF in water-based and human plasmasamples by using a high-performance liquid chromatography method with ultraviolet detection (HPLC-UV). The analyte wasextracted from 0.25 mL water or human plasma samples with an acetonitrile-based solution and analyzed on a C18 reversedphasecolumn. Sorafenib was used as an internal standard (IS). The mobile phase was composed of ammonium acetatebuffer, and methanol and acetonitrile. The flow rate was 1.0 mL/min, and the UV wavelength was set at 265 nm. Theretention times of SOF and IS were 7.8 min and 9.1 min, respectively. The total run time for a single analysis was 15.0 min.The calibration curve was linear within the range 0.1-10.0 μg/mL. The limit of quantitation was 0.1 μg/mL. The recovery ofthe drug from human plasma samples was greater than 99 %. The intra- and inter-day precision of water-based and plasmasamples ranged from 1.41% to 10.06% and 0.21 to 6.53%, respectively. The intra- and inter-day accuracy of water-based andplasma samples ranged from 92.71 to 98.41% and 89.31 to 101.21%, respectively. The proposed method is very simple andallows acquiring a good recovery of the analytes. The developed HPLC-UV method may be applied to conductingpharmacokinetic studies in the determination of SOF.

収録刊行物

  • 国際医療福祉大学学会誌

    国際医療福祉大学学会誌 23(1), 130-136, 2018

    国際医療福祉大学学会

各種コード

  • NII論文ID(NAID)
    120006461263
  • NII書誌ID(NCID)
    AA12537130
  • 本文言語コード
    ENG
  • 資料種別
    journal article
  • 雑誌種別
    大学紀要
  • ISSN
    2186-3652
  • NDL 記事登録ID
    029014657
  • NDL 請求記号
    Z19-B929
  • データ提供元
    NDL  IR 
ページトップへ