Single-Molecule Manipulation of the Duplex Formation and Dissociation at the G-Quadruplex/i-Motif Site in the DNA Nanostructure

• 遠藤, 政幸

  Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University・CREST, Japan Science and Technology Corporation (JST)

• 江村, 智子

  Department of Chemistry, Graduate School of Science, Kyoto University・College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University

• 日高, 久美

  College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University

• 杉山, 弘

  Department of Chemistry, Graduate School of Science, Kyoto University

• K, Hidaka

  Department of Chemistry, Graduate School of Science, Kyoto University

• B, Tuesuwan

  Department of Food and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University

• H, Sugiyama

  Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University・Department of Chemistry, Graduate School of Science, Kyoto University・CREST, Japan Science and Technology Corporation (JST)

抄録

We demonstrate the single-molecule operation and observation of the formation and resolution of double-stranded DNA (dsDNA) containing a G-quadruplex (GQ) forming and counterpart i-motif forming sequence in the DNA nanostructure. Sequential manipulation of DNA strands in the DNA frame was performed to prepare a topologically controlled GQ/i-motif dsDNA. Using strand displacement and the addition and removal of K+, the topologically controlled GQ/i-motif dsDNA in the DNA frame was obtained in high yield. The dsDNA was resolved into the single-stranded DNA, GQ, and i-motif by the addition of K+ and operation in acidic conditions. The dissociation of the dsDNA under the GQ and i-motif formation condition was monitored by high-speed atomic force microscopy. The results indicate that the dsDNA containing the GQ- and i-motif sequence is effectively dissolved when the duplex is helically loosened in the DNA nanoscaffold.

収録刊行物

• ACS nano

  ACS nano 9 (10), 9922-9929, 2015-10-27

  American Chemical Society (ACS)

キーワード

• DNA origami
• G-quadruplex
• high-speed AFM
• i-motif
• single-molecule observation

詳細情報

• CRID: 1050564285810908544
• NII論文ID: 120006462492
• ISSN: 19360851
• ISSN: 1936086X
• DOI: 10.1021/acsnano.5b03413
• HANDLE: 2433/230920
• Web Site: https://pubs.acs.org/doi/pdf/10.1021/acsnano.5b03413
• 本文言語コード: en
• 資料種別: journal article
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