Human AK2 links intracellular bioenergetic redistribution to the fate of hematopoietic progenitors

• Oshima, Koichi

• Saiki, Norikazu

• Tanaka, Michihiro

• Imamura, Hiromi

• Niwa, Akira

• Tanimura, Ayako

• Nagahashi, Ayako

• Hirayama, Akiyoshi

• Okita, Keisuke

• Hotta, Akitsu

• Kitayama, Shuichi

• Osawa, Mitsujiro

• Kaneko, Shin

• Watanabe, Akira

• Asaka, Isao

• Fujibuchi, Wataru

• Imai, Kohsuke

• Yabe, Hiromasa

• Kamachi, Yoshiro

• Hara, Junichi

• Kojima, Seiji

• Tomita, Masaru

• Soga, Tomoyoshi

• Noma, Takafumi

• Nonoyama, Shigeaki

• Nakahata, Tatsutoshi

• Saito, Megumu K.

抄録

AK2 is an adenylate phosphotransferase that localizes at the intermembrane spaces of the mitochondria, and its mutations cause a severe combined immunodeficiency with neutrophil maturation arrest named reticular dysgenesis (RD). Although the dysfunction of hematopoietic stem cells (HSCs) has been implicated, earlier developmental events that affect the fate of HSCs and/or hematopoietic progenitors have not been reported. Here, we used RD-patient-derived induced pluripotent stem cells (iPSCs) as a model of AK2-deficient human cells. Hematopoietic differentiation from RD-iPSCs was profoundly impaired. RD-iPSC-derived hemoangiogenic progenitor cells (HAPCs) showed decreased ATP distribution in the nucleus and altered global transcriptional profiles. Thus, AK2 has a stage-specific role in maintaining the ATP supply to the nucleus during hematopoietic differentiation, which affects the transcriptional profiles necessary for controlling the fate of multipotential HAPCs. Our data suggest that maintaining the appropriate energy level of each organelle by the intracellular redistribution of ATP is important for controlling the fate of progenitor cells.

収録刊行物

• Biochemical and Biophysical Research Communications

  Biochemical and Biophysical Research Communications 497 (2), 719-725, 2018-02-17

  Elsevier

キーワード

• Induced pluripotent stem cells
• Adenylate kinase 2
• Hemoangiogenic progenitor cells
• Phosphotransfer

詳細情報

• CRID: 1050004225273510400
• NII論文ID: 120006466601
• NII書誌ID: AA00564395
• ISSN: 0006291X
• DOI: 10.1016/j.bbrc.2018.02.139
• HANDLE: 2433/231081
• Web Site: http://repo.lib.tokushima-u.ac.jp/115015
• Web Site: https://api.elsevier.com/content/article/PII:S0006291X1830370X?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S0006291X1830370X?httpAccept=text/plain
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN