Inverse tissue mechanics of cell monolayer expansion

• 近藤, 洋平

  Graduate School of Informatics, Kyoto University・Division of Quantitative Biology, National Institute for Basic Biology・Department of Basic Biology, Faculty of Life Science, SOKENDAI (Graduate University for Advanced Studies)

• 石井, 信

  Division of Quantitative Biology, National Institute for Basic Biology・Department of Basic Biology, Faculty of Life Science, SOKENDAI (Graduate University for Advanced Studies)

• Ishii, Shin

  Graduate School of Informatics, Kyoto University

• Philip K Maini

  editor

抄録

Living tissues undergo deformation during morphogenesis. In this process, cells generate mechanical forces that drive the coordinated cell motion and shape changes. Recent advances in experimental and theoretical techniques have enabled in situ measurement of the mechanical forces, but the characterization of mechanical properties that determine how these forces quantitatively affect tissue deformation remains challenging, and this represents a major obstacle for the complete understanding of morphogenesis. Here, we proposed a non-invasive reverse-engineering approach for the estimation of the mechanical properties, by combining tissue mechanics modeling and statistical machine learning. Our strategy is to model the tissue as a continuum mechanical system and to use passive observations of spontaneous tissue deformation and force fields to statistically estimate the model parameters. This method was applied to the analysis of the collective migration of Madin-Darby canine kidney cells, and the tissue flow and force were simultaneously observed by the phase contrast imaging and traction force microscopy. We found that our monolayer elastic model, whose elastic moduli were reverse-engineered, enabled a long-term forecast of the traction force fields when given the tissue flow fields, indicating that the elasticity contributes to the evolution of the tissue stress. Furthermore, we investigated the tissues in which myosin was inhibited by blebbistatin treatment, and observed a several-fold reduction in the elastic moduli. The obtained results validate our framework, which paves the way to the estimation of mechanical properties of living tissues during morphogenesis.

収録刊行物

• PLOS Computational Biology

  PLOS Computational Biology 14 (3), e1006029-, 2018-03-01

  Public Library of Science (PLoS)

キーワード

• Computational Theory and Mathematics
• Cellular and Molecular Neuroscience
• Genetics
• Molecular Biology
• Ecology
• Modeling and Simulation
• Ecology, Evolution, Behavior and Systematics

詳細情報

• CRID: 1050845760787656320
• NII論文ID: 120006466609
• ISSN: 15537358
• DOI: 10.1371/journal.pcbi.1006029
• HANDLE: 2433/231089
• Web Site: http://dx.plos.org/10.1371/journal.pcbi.1006029
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN