Chemical hijacking of auxin signaling with an engineered auxin–TIR1 pair

• Uchida, Naoyuki

• Takahashi, Koji

• Iwasaki, Rie

• Yamada, Ryotaro

• Yoshimura, Masahiko

• Endo, Takaho A

• Kimura, Seisuke

• Zhang, Hua

• Nomoto, Mika

• Tada, Yasuomi

• Kinoshita, Toshinori

• Itami, Kenichiro

• Hagihara, Shinya

• Torii, Keiko U

Abstract

The phytohormone auxin indole-3-acetic acid (IAA) regulates nearly all aspects of plant growth and development. Despite substantial progress in our understanding of auxin biology, delineating specific auxin response remains a major challenge. Auxin regulates transcriptional response via its receptors, TIR1 and AFB F-box proteins. Here we report an engineered, orthogonal auxin–TIR1 receptor pair, developed through a bump-and-hole strategy, that triggers auxin signaling without interfering with endogenous auxin or TIR1/AFBs. A synthetic, convex IAA (cvxIAA) hijacked the downstream auxin signaling in vivo both at the transcriptomic level and in specific developmental contexts, only in the presence of a complementary, concave TIR1 (ccvTIR1) receptor. Harnessing the cvxIAA–ccvTIR1 system, we provide conclusive evidence for the role of the TIR1-mediated pathway in auxin-induced seedling acid growth. The cvxIAA–ccvTIR1 system serves as a powerful tool for solving outstanding questions in auxin biology and for precise manipulation of auxin-mediated processes as a controllable switch.

Journal

• Nature Chemical Biology

  Nature Chemical Biology 14 (3), 299-305, 2018-01-22

  nature

Keywords

• Cell Biology
• Molecular Biology

Details

• CRID: 1050001338806823296
• NII Article ID: 120006473593
• DOI: 10.1038/nchembio.2555
• ISSN: 15524469
• ISSN: 15524450
• HANDLE: 2237/00028291
• Web Site: https://nagoya.repo.nii.ac.jp/records/26086
• Web Site: http://www.nature.com/articles/nchembio.2555.pdf
• Web Site: http://www.nature.com/articles/nchembio.2555
• Web Site: http://www.nature.com/doifinder/10.1038/nchembio.2555
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN