Vibrio alginolyticus VepA Induces Lysosomal Membrane Permeability and Cathepsin-Independent Cell Death

• Darwinata Agus Eka

• Gotoh Kazuyoshi

• Mima Takehiko

• Yamamoto Yumiko

• Yokota Kenji

• Matsushita Osamu

Abstract

The bacterium Vibrio alginolyticus, an opportunistic pathogen in humans, has a type III secretion system (T3SS) that is responsible for its cytotoxicity toward eukaryotic cells. The effector of T3SS that is responsible for the cytotoxicity had not been identified. Here we demonstrate that VepA, a homolog of the T3SS effector in V. parahaemolyticus, is required for cytotoxicity in V. alginolyticus. VepA induces lysosomal membrane permeabilization, and it allows the leakage of only small molecules into the cytosol. Our findings revealed that VepA induces cathepsin-independent cell death in mammalian cells. The ferrous ion, one of the small molecules in the lysosome contents, appears to be involved in the cell death caused by V. alginolyticus VepA.

Journal

• Acta Medica Okayama

  Acta Medica Okayama 72 (3), 231-239, 2018-06

  Okayama University Medical School

Keywords

• cell death
• lysosomal membrane permeability
• VepA
• Vibrio alginolyticus

Details

• CRID: 1390572174774505856
• NII Article ID: 120006482817
• NII Book ID: AA00508441
• ISSN: 0386300X
• DOI: 10.18926/amo/56068
• PubMed: 29926000
• Web Site: https://ousar.lib.okayama-u.ac.jp/56068
• Web Site: https://search.jamas.or.jp/link/ui/2019024723
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • PubMed
  • CiNii Articles
  • KAKEN