An N-sulfanylethylanilide-based traceable linker for enrichment and selective labelling of target proteins

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Abstract

An N-sulfanylethylanilide-based traceable linker, developed to facilitate identification of target proteins of bioactive compounds, was introduced into an alkynylated target protein. Subsequent adsorption onto streptavidin beads allowed it to be treated with a cysteine-fluorophore conjugate in the presence of phosphate. This induced the N-S acyl transfer reaction of the N-sulfanylethylanilide unit. The subsequent native chemical ligation of the fluorophore resulted in cleavage of the linker for target elution and fluorescent labelling of the target, allowing it to be distinguished from non-target proteins.

Journal

  • Chemical Communications

    Chemical Communications 52(42), 6911-6913, 2016-04-26

    The Royal Society of Chemistry

Codes

  • NII Article ID (NAID)
    120006493363
  • NII NACSIS-CAT ID (NCID)
    AA11071130
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    1359-7345
  • Data Source
    IR 
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