Attenuated lipopolysaccharide-induced inflammatory bladder hypersensitivity in mice deficient of transient receptor potential ankilin1

• 中川, 貴之

  Department of Continence Medicine, The University of Tokyo Graduate School of Medicine･Department of Urology, The University of Tokyo Graduate School of Medicine

• 金子, 周司

  Department of Continence Medicine, The University of Tokyo Graduate School of Medicine

• Nakagawa, Takayuki

  Department of Clinical Pharmacology and Therapeutics, Kyoto University Hospital･Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University

• Kaneko, Shuji

  Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University

• Kume, Haruki

  Department of Urology, The University of Tokyo Graduate School of Medicine

• Homma, Yukio

  Department of Urology, The University of Tokyo Graduate School of Medicine･Department of Urology, Japan Red Cross Medical Center

• Igawa, Yasuhiko

  Department of Continence Medicine, The University of Tokyo Graduate School of Medicine

抄録

Transient receptor potential ankyrin 1 (TRPA1) channel expressed by urothelial cells and bladder sensory nerve fibers might act as a bladder mechanosensor and nociceptive transducer. To disclose the role of TRPA1 in bladder function and inflammation-associated hypersensitivity, we evaluated in vitro and in vivo bladder function and inflammatory mechanosensory and nociceptive responses to intravesical lipopolysaccharide (LPS)-instillation in wild type (WT) and TRPA1-knock out (KO) mice. At baseline before treatment, no significant differences were observed in frequency volume variables, in vitro detrusor contractility, and cystometric parameters between the two groups in either sex. LPS-instillation significantly increased voiding frequency and decreased mean voided volume at 24–48 hours after instillation in WT but not in TRPA1-KO mice. LPS-instillation also significantly increased the number of pain-like behavior at 24 hours after instillation in WT mice, but not in TRPA1-KO mice. Cystometry 24 hours after LPS-instillation revealed shorter inter-contraction intervals in the WT mice compared with TRPA1-KO mice. In contrast, inflammatory cell infiltration in the bladder suburothelial layer was not significantly different between the two groups. These results indicate that TRPA1 channels are involved in bladder mechanosensory and nociceptive hypersensitivity accompanied with inflammation but not in physiological bladder function or development of bladder inflammation.

収録刊行物

• Scientific reports

  Scientific reports 8 2018-10-23

  Springer Nature America, Inc

キーワード

• Multidisciplinary

詳細情報

• CRID: 1050001338207758848
• NII論文ID: 120006534589
• ISSN: 20452322
• DOI: 10.1038/s41598-018-33967-x
• HANDLE: 2433/234935
• Web Site: https://www.nature.com/articles/s41598-018-33967-x.pdf
• Web Site: https://www.nature.com/articles/s41598-018-33967-x
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN