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Abstract

Marchantia polymorpha is one of the model species of basal land plants. Although CRISPR/ Cas9-based genome editing has already been demonstrated for this plant, the efficiency was too low to apply to functional analysis. In this study, we show the establishment of CRISPR/Cas9 genome editing vectors with high efficiency for both construction and genome editing. Codon optimization of Cas9 to Arabidopsis achieved over 70% genome editing efficiency at two loci tested. Systematic assessment revealed that guide sequences of 17 nt or shorter dramatically decreased this efficiency. We also demonstrated that a combinatorial use of this system and a floxed complementation construct enabled conditional analysis of a nearly essential gene. This study reports that simple, rapid, and efficient genome editing is feasible with the series of developed vectors.

Journal

  • PLOS ONE

    PLOS ONE 13(10), e0205117, 2018-10-31

    PLOS

Codes

  • NII Article ID (NAID)
    120006534595
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    1932-6203
  • Data Source
    IR 
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