Protein kinase D regulates positive selection of CD4+ thymocytes through phosphorylation of SHP-1

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Abstract

Thymic selection shapes an appropriate T cell antigen receptor (TCR) repertoire during T cell development. Here, we show that a serine/threonine kinase, protein kinase D (PKD), is crucial for thymocyte positive selection. In T cell-specific PKD-deficient (PKD2/PKD3 double-deficient) mice, the generation of CD4 single positive thymocytes is abrogated. This defect is likely caused by attenuated TCR signalling during positive selection and incomplete CD4 lineage specification in PKD-deficient thymocytes; however, TCR-proximal tyrosine phosphorylation is not affected. PKD is activated in CD4+CD8+ double positive (DP) thymocytes on stimulation with positively selecting peptides. By phosphoproteomic analysis, we identify SH2-containing protein tyrosine phosphatase-1 (SHP-1) as a direct substrate of PKD. Substitution of wild-type SHP-1 by phosphorylation-defective mutant (SHP-1S557A) impairs generation of CD4+ thymocytes. These results suggest that the PKD–SHP-1 axis positively regulates TCR signalling to promote CD4+ T cell development.

Journal

  • Nature Communications

    Nature Communications (7), 12756, 2016-09-27

    Springer Nature

Codes

  • NII Article ID (NAID)
    120006535062
  • NII NACSIS-CAT ID (NCID)
    AA12645905
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    2041-1723
  • Data Source
    IR 
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