<scp>RFTS</scp>‐dependent negative regulation of Dnmt1 by nucleosome structure and histone tails

• Mishima, Yuichi

• Brueckner, Laura

• Takahashi, Saori

• Kawakami, Toru

• Arita, Kyohei

• Oka, Shota

• 大谷, 淳二

• Hojo, Hironobu

• Shirakawa, Masahiro

• Shinohara, Akira

• Watanabe, Mikio

• Suetake, Isao

書誌事項

タイトル別名

• RFTS-dependent negative regulation of Dnmt1 by nucleosome structure and histone tails

抄録

DNA methylation in promoter regions represses gene expression and is copied over mitotic divisions by Dnmt1. Dnmt1 activity is regulated by its replication foci targeting sequence (RFTS) domain which masks the catalytic pocket. It has been shown that Dnmt1 activity on unmethylated DNA is inhibited in nucleosome cores. In the present study, we aimed to assess the effect of nuclesome formation on maintenance methylation at single CpG resolution. We show that Dnmt1 fully methylates naked linker DNA in dinucleosomes, whereas maintenance methylation was repressed at all CpG sites in nucleosome core particles. Deletion of RFTS partly released obstruction of Dnmt1 activity in core particles. Histone H3 tail peptides inhibited Dnmt1 in an RFTS‐dependent manner and repression was modulated by acetylation or methylation. We propose a novel function of RFTS to regulate Dnmt1 activity in nucleosomes.

収録刊行物

• FEBS Journal

  FEBS Journal 284 (20), 3455-3469, 2017-10

  Wiley

キーワード

• Dnmt1
• histone
• modifications
• nucleosomes
• replication foci targeting sequence

詳細情報

• CRID: 1050012570392981760
• NII論文ID: 120006537075
• NII書誌ID: AA11998513
• DOI: 10.1111/febs.14205
• ISSN: 17424658
• ISSN: 1742464X
• HANDLE: 20.500.14094/90005851
• HANDLE: 2433/235000
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Ffebs.14205
• Web Site: https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/febs.14205
• 本文言語コード: en
• 資料種別: journal article
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