Engineering of perfusable double-layered vascular structures using contraction of spheroid-embedded hydrogel and electrochemical cell detachment
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Abstract
Perfusable vasculatures are essential for engineering three-dimensional thick tissues and organs in the fields of tissue engineering and regenerative medicine. Here, we describe an approach for the fabrication of double-layered vascular-like structures (DVSs) composed of a monolayer of human vascular endothelial cells (HUVECs) covered with a dense human smooth muscle cell (SMC) layer. HUVECs were attached to a gold needle via the oligopeptide self-assembled monolayer and grown to form a HUVEC monolayer that was subsequently embedded in a photo-crosslinkable gelatin hydrogel containing SMC spheroids in a culture chamber. During four days of culture, the hydrogel significantly contracted and formed a dense SMC layer around the needle. The binding between the HUVEC layer and the gold needle was cleaved by applying a negative potential to desorb the oligopeptide and the needle was extracted from the chamber, resulting in a perfusable DVS composed of HUVEC and SMC layers. The DVS was cultured under perfusion, and the cells in the DVS showed greater expressions of SMC-specific genes compared to those of spheroids. The DVS possessed a dynamic contraction ability in response to acetylcholine as observed in the in vivo SMC layer. This study proposes a promising approach for the fabrication of perfusable vasculatures for the engineering of fully vascularized tissues and organs.
Journal
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- Journal of Bioscience and Bioengineering
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Journal of Bioscience and Bioengineering 127 (1), 114-120, 2019-01
Elsevier B.V.
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Details 詳細情報について
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- CRID
- 1050001337834941312
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- NII Article ID
- 120006539323
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- NII Book ID
- AA11307678
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- ISSN
- 13891723
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- NDL BIB ID
- 029459309
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- Web Site
- http://hdl.handle.net/10131/00012076
- http://id.ndl.go.jp/bib/029459309
- https://ndlsearch.ndl.go.jp/books/R000000004-I029459309
- https://api.elsevier.com/content/article/PII:S1389172318303293?httpAccept=text/xml
- https://api.elsevier.com/content/article/PII:S1389172318303293?httpAccept=text/plain
- https://search.jamas.or.jp/link/ui/2019253890
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- IRDB
- NDL
- Crossref
- CiNii Articles
- KAKEN