Enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe

• Takayama, Seiya

• Ozaki, Aiko

• Konishi, Rie

• Otomo, Chisako

• Kishida, Mayumi

• Hirata, Yuuki

• 松本, 拓也

• 田中, 勉

• 近藤, 昭彦

抄録

Background Economical production of value-added chemicals from renewable biomass is a promising path to sustainability. 3-Hydroxypropionic acid (3-HP) is an important chemical for building a bio-sustainable society. Establishment of 3-HP production from renewable resources such as glucose would provide a bio-sustainable alternative to the production of acrylic acid from fossil resources. Results Here, we describe metabolic engineering of the fission yeast Schizosaccharomyces pombe to enhance 3-HP production from glucose and cellobiose via the malonyl-CoA pathway. The mcr gene, encoding the malonyl-CoA reductase of Chloroflexus aurantiacus, was dissected into two functionally distinct fragments, and the activities of the encoded protein were balanced. To increase the cellular supply of malonyl-CoA and acetyl-CoA, we introduced genes encoding endogenous aldehyde dehydrogenase, acetyl-CoA synthase from Salmonella enterica, and endogenous pantothenate kinase. The resulting strain produced 3-HP at 1.0g/L from a culture starting at a glucose concentration of 50g/L. We also engineered the sugar supply by displaying beta-glucosidase (BGL) on the yeast cell surface. When grown on 50g/L cellobiose, the beta-glucosidase-displaying strain consumed cellobiose efficiently and produced 3-HP at 3.5g/L. Under fed-batch conditions starting from cellobiose, this strain produced 3-HP at up to 11.4g/L, corresponding to a yield of 11.2% (g-3-HP/g-glucose; given that 1g cellobiose corresponds to 1.1g glucose upon digestion). Conclusions In this study, we constructed a series of S. pombe strains that produced 3-HP via the malonyl-CoA pathway. Our study also demonstrated that BGL display using cellobiose and/or cello-oligosaccharides as a carbon source has the potential to improve the titer and yield of malonyl-CoA- and acetyl-CoA-derived compounds.

収録刊行物

• Microbial Cell Factories

  Microbial Cell Factories 17 176-176, 2018-11-13

  BMC

キーワード

• Schizosaccharomyces pombe
• 3-Hydroxypropionic acid
• CRISPR-Cas9 system
• Cell surface display
• Malonyl-CoA

詳細情報

• CRID: 1050294045369871488
• NII論文ID: 120006540096
• DOI: 10.1186/s12934-018-1025-5
• ISSN: 14752859
• HANDLE: 20.500.14094/90005399
• Web Site: http://link.springer.com/content/pdf/10.1186/s12934-018-1025-5.pdf
• Web Site: http://link.springer.com/article/10.1186/s12934-018-1025-5/fulltext.html
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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