A simple method to detect the tandem repeat of the cyp51A promoter in azole-resistant strains of Aspergillus fumigatus

• 豊留, 孝仁

• Hagiwara, Daisuke

• Watanabe, Akira

• Kamei, Katsuhiko

抄録

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We designed primers and cycling probes to detect the tandem repeat (TR) of cyp51A promoter in Aspergillus fumigatus. A control-probe was designed to anneal to the outside of the TR region, whereas a TR-probe was designed to anneal to the inside of the TR region. For amplification and probe-hydrolysis detection, the CycleavePCR system was used. Although the difference between Ct values of the wild-type genome for the control-probe and the TR-probe was around -0.1, the difference between Ct values of TR-harboring strains was around 0.7. These data indicate that this is a simple method to detect TR in azole-resistant A. fumigatus.

収録刊行物

• Medical mycology

  Medical mycology 56 (8), 1042-1044, 2018-11

  Oxford University Press

キーワード

• real-time PCR
• cycling probe
• Aspergillus fumigatus
• tandem repeat
• azole resistance

詳細情報

• CRID: 1050845762742321408
• NII論文ID: 120006540777
• NII書誌ID: AA12422809
• ISSN: 14602709
• Web Site: https://obihiro.repo.nii.ac.jp/records/4296
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles