A simple method to detect the tandem repeat of the cyp51A promoter in azole-resistant strains of Aspergillus fumigatus

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Abstract

We designed primers and cycling probes to detect the tandem repeat (TR) of cyp51A promoter in Aspergillus fumigatus. A control-probe was designed to anneal to the outside of the TR region, whereas a TR-probe was designed to anneal to the inside of the TR region. For amplification and probe-hydrolysis detection, the CycleavePCR system was used. Although the difference between Ct values of the wild-type genome for the control-probe and the TR-probe was around -0.1, the difference between Ct values of TR-harboring strains was around 0.7. These data indicate that this is a simple method to detect TR in azole-resistant A. fumigatus.

Journal

  • Medical mycology

    Medical mycology 56(8), 1042-1044, 2018-11

    Oxford University Press

Codes

  • NII Article ID (NAID)
    120006540777
  • NII NACSIS-CAT ID (NCID)
    AA12422809
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    1460-2709
  • Data Source
    IR 
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