Pantothenate auxotrophy of <i>Methylobacterium</i> spp. isolated from living plants

• 吉田, 裕介

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• 由里本, 博也

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University･Department of Agriculture and Food Technology, Faculty of Bioenvironmental Science, Kyoto Gakuen University

• Sakai, Yasuyoshi

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• Yurimoto, Hiroya

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University /

書誌事項

タイトル別名

• Pantothenate auxotrophy of Methylobacterium spp. isolated from living plants

抄録

A number of pink-pigmented facultative methylotrophs (PPFMs) belonging to Methylobacterium spp. isolated from living plant samples were found to require B vitamins for their growth in minimal medium, and most B vitamin-auxotrophic PPFMs required pantothenate (vitamin B5). Further investigation of pantothenate auxotrophy using the representative strain Methylobacterium sp. OR01 demonstrated that this strain cannot synthesize β-alanine, one of the precursors of pantothenate. β-alanine and several precursors of pantothenate restored the growth of Methylobacterium sp. OR01 in minimal medium. Furthermore, this strain could colonize leaves of Arabidopsis thaliana cultivated in medium without pantothenate or its precursors. Pantothenate, β-alanine and several precursors were detected in the suspension of A. thaliana leaves. These results suggest that pantothenate-auxotrophic PPFMs can symbiotically colonize the surface of plant leaves by acquiring β-alanine and other precursors, in addition to pantothenate. Finally, the fitness advantage of B vitamin auxotrophy of PPFMs in the phyllosphere environment is discussed. Pantothenate-auxotrophic strains of Methylobacterium show symbiotic colonization on plants through acquisition of pantothenate and its precursors.

収録刊行物

• Bioscience, biotechnology, and biochemistry

  Bioscience, biotechnology, and biochemistry 83 (3), 569-577, 2018-11-26

  Informa UK Limited

キーワード

• B vitamin auxotrophy
• pantothenate
• β-alanine
• Methylobacterium
• phyllosphere

詳細情報

• CRID: 1050282813879277440
• NII論文ID: 120006543408
• ISSN: 09168451
• ISSN: 13476947
• DOI: 10.1080/09168451.2018.1549935
• HANDLE: 2433/235623
• Web Site: http://academic.oup.com/bbb/article-pdf/83/3/569/36826283/bbb0569.pdf
• 本文言語コード: en
• 資料種別: journal article
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