Successful cryopreservation of spermatogonia in critically endangered Manchurian trout (Brachymystax lenok)

• Lee, Seungki

• Yoshizaki, Goro

抄録

Because of the lack of cryopreservation techniques for fish eggs and embryos, 22 cryopreservation of fish spermatogonia and subsequent generation of eggs and sperm would be 23 an exit strategy for the long-term preservation of genetic resources. This study aimed to optimize 24 cryoprotectants, cooling rates, and thawing temperatures for slow freezing of spermatogonia from 25 endangered Manchurian trout (Brachymystax lenok). Whole testes were frozen with a 26 cryomedium containing 1.3 M methanol, 0.2 M trehalose, and 10% egg yolk at a cooling rate of 27 −1°C/min and then stored in liquid nitrogen for 2 days. After thawing at 30°C in a water bath, 28 testicular cells from thawed testes were intraperitoneally transplanted into allogeneic triploid 29 hatchlings. Transplanted spermatogonia migrated toward and were incorporated into recipient 30 gonads, where they underwent gametogenesis. Transplantation efficiency did not significantly 31 differ between frozen and fresh testes, demonstrating that Manchurian trout spermatogonia can be 32 successfully cryopreserved in liquid nitrogen.

source:https://doi.org/10.1016/j.cryobiol.2016.01.004

source:https://kaken.nii.ac.jp/ja/grant/KAKENHI-PLANNED-25114005/

source:https://nrid.nii.ac.jp/ja/nrid/1000070281003/

identifier:25114005

収録刊行物

• Cryobiology

  Cryobiology 72 (2), 165-168, 2016-04

  Elsevier

キーワード

• Manchurian trout
• Cryopreservation
• Slow freezing
• Spermatogonial transplantation
• Spermatogonial stem cells
• 科学研究費研究成果
• サケ科魚類の進化に伴うGSC制御機構の変化
• Evolution of GSC regulation in salmonids Planned

詳細情報

• CRID: 1050282677548180096
• NII論文ID: 120006547603
• NII書誌ID: AA00620601
• ISSN: 00112240
• Web Site: http://id.nii.ac.jp/1342/00001602/
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles
  • KAKEN