Development of a reinforced Ti-eviction plasmid useful for construction of Ti plasmid-free Agrobacterium strains

IR Open Access

Abstract

For curing stable Ti plasmids from Agrobacterium strains, we developed a new Ti-eviction plasmid. The plasmid contains two antitoxin genes, which originated from two toxin-antitoxin systems in Ti plasmids. The plasmid facilitated production of Ti-less cells in seven tested strains, and was removed easily by simple counter-selection from the cells.

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