Hyperpolarization by N-(3-oxododecanoyl)-L-homoserine-lactone, a quorum sensing molecule, in rat thymic lymphocytes

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Abstract

To study the adverse effects of N-(3-oxododecanoyl)-L-homoserine-lactone (ODHL), a quorum sensing molecule, on mammalian host cells, its effect on membrane potential was examined in rat thymic lymphocytes using flow cytometric techniques with a voltage-sensitive fluorescent probe. As 3–300 μM ODHL elicited hyperpolarization, it is likely that it increases membrane K+ permeability because hyperpolarization is directly linked to changing K+ gradient across membranes, but not Na+ and Cl- gradients. ODHL did not increase intracellular Ca2+ concentration. ODHL also produced a response in the presence of an intracellular Zn2+ chelator. Thus, it is unlikely that intracellular Ca2+ and Zn2+ are attributed to the response. Quinine, a non-specific K+ channel blocker, greatly reduced hyperpolarization. However, because charybdotoxin, tetraethylammonium chloride, 4-aminopyridine, and glibenclamide did not affect it, it is pharmacologically hypothesized that Ca2+-activated K+ channels, voltage-gated K+ channels, and ATP-sensitive K+ channels are not involved in ODHL-induced hyperpolarization. Although the K+ channels responsible for ODHL-induced hyperpolarization have not been identified, it is suggested that ODHL can elicit hyperpolarization in mammalian host cells, disturbing cellular functions.

Journal

  • Chemico-Biological Interactions

    Chemico-Biological Interactions (283), 91-96, 2018-02-08

    Elsevier

Codes

  • NII Article ID (NAID)
    120006556226
  • NII NACSIS-CAT ID (NCID)
    AA0060252X
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    0009-2797
  • Data Source
    IR 
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