Nanofluidic Biosensor Created by Bonding Patterned Model Cell Membrane and Silicone Elastomer with Silica Nanoparticles

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Abstract

Selective and sensitive detection of specific molecules in a solution containing diverse coexisting molecules is important in many biomedical and environmental applications, including diagnostics and pollutant detection. Here, a nanofluidic biosensor is developed to detect specific target molecules (e.g., toxin proteins) in the presence of nontarget molecules by bonding a patterned model cell membrane and a silicone elastomer (polydimethylsiloxane: PDMS) sheet using surface-modified silica nanoparticles as the adhesive layer. Owing to the uniform size of nanoparticles, a nanometric gap junction is formed between the fluid bilayer and PDMS (nanogap-junction). The thickness of the nanogap-junction is controlled by the size of the silica nanoparticles. Target molecules that specifically bind to the receptor molecules in the fluid bilayer are selectively transported into the nanogap-junction via lateral diffusion through the lipid membrane. A thinner gap formed with smaller nanoparticles can enhance the sensitivity (signal-to-background ratio) more effectively, owing to the suppression of nonspecific penetration of coexisting molecules. Silica nanoparticles also provide excellent mechanical robustness, realizing long-term stability of the gap structure. Nanogap-junction using silica nanoparticles provides a versatile platform for highly selective and sensitive sensing by realizing detection of specific target molecules in a solution containing more concentrated nontarget molecules.

Journal

  • Small

    Small 14(49), 1802804, 2018-12-06

    Wiley‐VCH Verlag

Codes

  • NII Article ID (NAID)
    120006559302
  • NII NACSIS-CAT ID (NCID)
    AA11975212
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    1613-6810
  • Data Source
    IR 
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