Comparative evaluations of lignocellulose reactivity and usability in transgenic rice plants with altered lignin composition

• Takeda, Yuri

  Research Institute for Sustainable Humanosphere, Kyoto University

• Tobimatsu, Yuki

  Research Institute for Sustainable Humanosphere, Kyoto University

• Yamamura, Masaomi

  Research Institute for Sustainable Humanosphere, Kyoto University

• Takano, Toshiyuki

  Graduate School of Agriculture, Kyoto University

• Sakamoto, Masahiro

  Graduate School of Agriculture, Kyoto University

• Umezawa, Toshiaki

  Research Institute for Sustainable Humanosphere, Kyoto University・Research Unit for Development of Global Sustainability, Kyoto University

Abstract

The aromatic composition of lignins is considered an important trait that affects the physico-chemical properties of lignocellulosic biomass. However, our knowledge of the relationship between lignin structure and biomass utilization properties remains limited, especially in monocotyledonous grass species, despite their potential as biomass feedstocks. In this study, we used recently produced rice transgenic lines with distinct lignin monomer compositions, i.e., guaiacyl (G)/syringyl (S)/p-hydroxyphenyl (H) aromatic unit ratios, to study the impact of lignin composition on the chemical reactivity, enzymatic saccharification efficiency and calorific value of rice lignocellulose. The H-lignin-enriched rice transgenic line showed significantly enhanced biomass saccharification efficiency after alkali and acid pretreatments and even without any pretreatment, whereas the S-lignin-enriched rice transgenic line displayed enhanced saccharification efficiency after liquid hot water pretreatment. While we detected no significant differences in biomass heating values between the transgenic rice materials tested, analysis of synthetic lignins comprising only G, S or H units suggested that increased ratios of G or H units could increase the heating value of lignin-based solid biofuels.

Journal

• Journal of Wood Science

  Journal of Wood Science 65 2019-02-13

  Springer Nature

Keywords

• p-Coumaroyl ester 3-hydroxylase
• Coniferaldehyde 5-hydroxylase
• Enzymatic saccharification
• Heating value
• Grass biomass

Details

• CRID: 1050845763139447808
• NII Article ID: 120006559417
• ISSN: 14350211
• ISSN: 16114663
• DOI: 10.1186/s10086-019-1784-6
• HANDLE: 2433/236641
• Web Site: http://link.springer.com/article/10.1186/s10086-019-1784-6/fulltext.html
• Web Site: http://link.springer.com/content/pdf/10.1186/s10086-019-1784-6.pdf
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN