Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification

• WATANABE, Takaaki

• TANABE, Hideyuki

• HORIUCHI, Takashi

抄録

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Gene amplification contributes to a variety of biological phenomena, including malignant progression and drug resistance. However, details of the molecular mechanisms remain to be determined. Here, we have developed a gene amplification system in yeast and mammalian cells that is based on double rolling-circle replication (DRCR). Cre-lox system is used to efficiently induce DRCR utilizing a recombinational process coupled with replication. This system shows distinctive features seen in amplification of oncogenes and drug-resistance genes: (i) intra- and extrachromosomal amplification, (ii) intensive chromosome rearrangement and (iii) scattered-type amplification resembling those seen in cancer cells. This system can serve as a model for amplification of oncogenes and drug-resistance genes, and improve amplification systems used for making pharmaceutical proteins in mammalian cells.

収録刊行物

• Nucleic Acids Research

  Nucleic Acids Research 39 (16), e106-, 2011-09

  Oxford University Press

詳細情報

• CRID: 1050282813904905344
• NII論文ID: 120006576055
• ISSN: 03051048
• Web Site: https://ir.soken.ac.jp/records/3957
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles