Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification
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Gene amplification contributes to a variety of biological phenomena, including malignant progression and drug resistance. However, details of the molecular mechanisms remain to be determined. Here, we have developed a gene amplification system in yeast and mammalian cells that is based on double rolling-circle replication (DRCR). Cre-lox system is used to efficiently induce DRCR utilizing a recombinational process coupled with replication. This system shows distinctive features seen in amplification of oncogenes and drug-resistance genes: (i) intra- and extrachromosomal amplification, (ii) intensive chromosome rearrangement and (iii) scattered-type amplification resembling those seen in cancer cells. This system can serve as a model for amplification of oncogenes and drug-resistance genes, and improve amplification systems used for making pharmaceutical proteins in mammalian cells.
- Nucleic Acids Research = Nucleic Acids Research
Nucleic Acids Research = Nucleic Acids Research 39(16), e106, 2011-09
Oxford University Press