Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification

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Abstract

Gene amplification contributes to a variety of biological phenomena, including malignant progression and drug resistance. However, details of the molecular mechanisms remain to be determined. Here, we have developed a gene amplification system in yeast and mammalian cells that is based on double rolling-circle replication (DRCR). Cre-lox system is used to efficiently induce DRCR utilizing a recombinational process coupled with replication. This system shows distinctive features seen in amplification of oncogenes and drug-resistance genes: (i) intra- and extrachromosomal amplification, (ii) intensive chromosome rearrangement and (iii) scattered-type amplification resembling those seen in cancer cells. This system can serve as a model for amplification of oncogenes and drug-resistance genes, and improve amplification systems used for making pharmaceutical proteins in mammalian cells.

Journal

  • Nucleic Acids Research = Nucleic Acids Research

    Nucleic Acids Research = Nucleic Acids Research 39(16), e106, 2011-09

    Oxford University Press

Codes

  • NII Article ID (NAID)
    120006576055
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    0305-1048
  • Data Source
    IR 
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