Isolation of viable but nonculturable Vibrio cholerae O1 from environmental water samples in Kolkata, India, in a culturable state

• Senoh, Mitsutoshi

• Ghosh-Banerjee, Jayeeta

• Mizuno, Tamaki

• Shinoda, Sumio

• Miyoshi, Shin-ichi

• Hamabata, Takashi

• Nair, G. Balakrish

• Takeda, Yoshifumi

抄録

Previously, we reported that viable but nonculturable (VBNC) Vibrio cholerae was converted into a culturable state by coculture with several eukaryotic cell lines including HT-29 cells. In this study, we found that a factor converting VBNC V. cholerae into a culturable state (FCVC) existed in cell extracts of eukaryotic cells. FCVC was nondialyzable, proteinase K-sensitive, and stable to heating at <60°C for 5 min. We prepared thiosulfate citrate bile salts sucrose (TCBS) plates with FCVC (F-TCBS plates). After confirming that VBNC V. cholerae O1 and O139 formed typical yellow colonies on F-TCBS plates, we tried to isolate cholera toxin gene-positive VBNC V. cholerae from environmental water samples collected in urban slum areas of Kolkata, India and succeeded in isolating V. cholerae O1 El Tor variant strains harboring a gene for the cholera toxin. The possible importance of VBNC V. cholerae O1 as a source of cholera outbreaks is discussed.

収録刊行物

• Microbiologyopen

  Microbiologyopen 3 (2), 239-246, 2014-02-18

  Wiley-Blackwell

キーワード

• Factor converting VBNC into culturable
• VBNC Vibrio cholerae

詳細情報

• CRID: 1050002213741550336
• NII論文ID: 120006627940
• ISSN: 20458827
• Web Site: https://ousar.lib.okayama-u.ac.jp/56674
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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  • CiNii Articles