Histone H3 is absent from organelle nucleoids in BY-2 cultured tobacco cells

• Takusagawa, Mari

• Tamotsu, Satoshi

• Sakai, Atsushi

Abstract

type:Article

The core histone proteins (H2A, H2B, H3, and H4) are nuclear-localized proteins that play a central role in the formation of nucleosome structure. They have long been considered to be absent from extra-nuclear, DNA-containing organelles; i.e., plastids and mitochondria. Recently, however, the targeting of core histone H3 to mitochondria, and the presence of nucleosome-like structures in mitochondrial nucleoids, were proposed in cauliflower and tobacco, respectively. Thus, we examined whether histone H3 was present in plant organelles and participated in the organization of nucleoid structure, using highly purified organelles and organelle nucleoids isolated from BY-2 cultured tobacco cells. Immuno-fluorescence microscopic observations and Western blotting analyses demonstrated that histone H3 was absent from organelles and organelle nucleoids, consistent with the historical hypothesis. Thus, the organization of organelle nucleoids, including putative nucleosome-like repetitive structures, should be constructed and maintained without participation of histone H3.

Journal

• Cell Biology International

  Cell Biology International 37 (7), 748-754, 2013-04

  John Wiley & Sons, Inc.

Keywords

• BY-2
• histone H3
• mitochondria
• nucleoids
• plastids
• tobacco

Details

• CRID: 1050001338391832064
• NII Article ID: 120006657672
• NII Book ID: AA11544414
• ISSN: 10656995
• ISSN: 10958355
• DOI: 10.1002/cbin.10091
• Web Site: http://hdl.handle.net/10935/3362
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcbin.10091
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1002/cbin.10091
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN