An immunohistochemical study of the expression of heat-shock protein-25 and cell proliferation in the dental pulp and enamel organ during odontogenesis in rat molars
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Objectives: The aim of this study is to clarify the functional significance of heat-shock protein (HSP)-25 during tooth development. Design: We compared the expression of HSP-25 in the dental epithelial and mesenchymal cells with their proliferative activity during odontogenesis in rat molars on postnatal Days 1–100 by immunohistochemistry using anti-HSP-25 and anti-5-bromo-2’-deoxyuridine (BrdU) for cell proliferation assay. Results: On Day one, BrdU-immunoreactive cells were densely located in the inner enamel epithelium in the cervical loop and inter-cusped areas and the dental pulp adjacent to them, whereas HSP-25-immunoractivity (IR) was restricted to the cusped area where odontoblasts and ameloblasts had already differentiated. Subsequently, BrdU-IR shifted in the apical direction to be localized around Hertwig’s epithelial root sheath during Days 5–30, never overlapping with concomitantly apically-shifted HSP-25-IR. On Days 60–100, BrdU-immunoreactive cells were hardly recognizable in the dental pulp, where HSP-25-IR was exclusively localized in the odontoblast layer. Furthermore, the odontoblast- and ameloblast-lineage cells exhibited two steps in the expression of HSP-25 throughout the postnatal stages: first, dental epithelial and pulpal mesenchymal cells showed a weak IR for HSP-25 after the cessation of their proliferative activity, and subsequently odontoblasts and ameloblasts consistently expressed an intense HSP-25-IR. Conclusion: Odontoblast- and ameloblast-lineage cells acquire HSP-25-IR after they complete their cell division, suggesting that this protein acts as a switch between cell proliferation and differentiation during tooth development. The consistent expression of HSP-25-IR in the formative cells may be involved in the maintenance of their functional integrity.
収録刊行物
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- Archives of Oral Biology
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Archives of Oral Biology 51 (5), 378-386, 2006-05
Elsevier
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詳細情報 詳細情報について
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- CRID
- 1050564289181804928
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- NII論文ID
- 120006735771
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- NII書誌ID
- AA00548457
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- ISSN
- 00039969
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- HANDLE
- 10191/1804
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- CiNii Articles