Analysis for genetic loci controlling protoscolex development in the Echinococcus multilocularis infection using congenic mice

• Islam, Md Atiqul

• Torigoe, Daisuke

• Kameda, Yayoi

• Irie, Takao

• Kouguchi, Hirokazu

• Nakao, Ryo

• Masum, Md Abdul

• Ichii, Osamu

• Kon, Yasuhiro

• Tag-EL-Din-Hassan, Hassan T.

• Morimatsu, Masami

• Yagi, Kinpei

• Agui, Takashi

抄録

The resistance/susceptibility to Echinococcus multilocularis infection in mice is genetically controlled. However, genetic factors responsible for these differences remain unknown. Our previous study in genetic linkage analysis has revealed that there is a significant quantitative trait locus (QTL) for the establishment of cyst (Emcysl), and a highly significant QTL for the development of protoscolex of E. multilocularis larvae (Empscl), on mouse chromosomes 6 and 1, respectively. The current study aimed to confirm these QTLs and narrow down the critical genetic region that controls resistance/susceptibility to E. multilocularis infection by establishing congenic and subcongenic lines from C57BL/6 (B6) and DBA/2 (D2) mice. For protoscolex development phenotype, two congenic lines, B6.D2-Empscl and D2.B6-Empscl were developed, where responsible QTL, Empsc1 was introgressed from D2 into B6 background and vice versa. For cyst establishment phenotype, two congenic lines, B6.D2-Emcys1 and D2.B6-Emcys1 were developed, where responsible QTL, Emcys1 was introgressed from D2 into B6 background and vice versa. Because there was no significant difference in cyst establishment between B6.D2Emcysl and D2.B6-Emcys1 mice after challenge with E. multilocularis, it is suggested that the Emcysl does not solely control the cyst establishment in mouse liver. However, infection experiments with B6.D2-Empscl and D2.B6-Empsc1 mice showed a significant difference in protoscolex development in the cyst. It confirms that the Empsc1 controls phenotype of the protoscolex development in the cyst. Subsequently, two subcongenic lines, B6.D2-Empsc1.1 and B6.D2-Empsc1.2 from B6.D2-Emcysl and one subcongenic line, D2.B6-Empsc1.1 from D2.B6-Empscl were developed to narrow down the critical region responsible for protoscolex development. From the results of infection experiments with E. multilocularis in these subcongenic mice, it is concluded that a gene responsible for protoscolex development is located between D1Mit290 (68.1 cM) and D1Mit511 (97.3 cM).

収録刊行物

• Infection, Genetics and Evolution

  Infection, Genetics and Evolution 65 65-71, 2018-11

  Elsevier

キーワード

• Echinococcus multilocularis
• Protoscolex
• Congenic mouse
• Quantitative trait locus

詳細情報

• CRID: 1050001339065179904
• NII論文ID: 120006764182
• DOI: 10.1016/j.meegid.2018.07.017
• HANDLE: 2115/76048
• ISSN: 15671348
• Web Site: https://api.elsevier.com/content/article/PII:S1567134818305161?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S1567134818305161?httpAccept=text/plain
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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