Modified nucleobase-specific gene regulation using engineered transcription activator-like effectors

• 今西, 未来

  Institute for Chemical Research, Kyoto University

• Imanishi, Miki

  Institute for Chemical Research, Kyoto University

抄録

Epigenetic modification, as typified by cytosine methylation, is a key aspect of gene regulation that affects many biological processes. However, the biological roles of individual methylated cytosines are poorly understood. Sequence-specific DNA recognition tools can be used to investigate the roles of individual instances of DNA methylation. Transcription activator-like effectors (TALEs), which are DNA-binding proteins, are promising candidate tools with designable sequence specificity and sensitivity to DNA methylation. In this review, we describe the bases of DNA recognition of TALEs, including methylated cytosine recognition, and the applications of TALEs for the study of methylated DNA. In addition, we discuss TALE-based epigenome editing and oxidized methylated cytosine recognition.

収録刊行物

• Advanced Drug Delivery Reviews

  Advanced Drug Delivery Reviews 147 59-65, 2019-07

  Elsevier B.V.

キーワード

• DNA binding
• DNA methylation
• Epigenetics
• Gene manipulation
• Gene targeting

詳細情報

• CRID: 1050845763835339008
• NII論文ID: 120006769207
• ISSN: 0169409X
• ISSN: 18728294
• HANDLE: 2433/244858
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles