Pig lacks functional NLRC4 and NAIP genes

• 佐久間, 智理

• 新開, 浩樹

• 竹之内, 敬人

• 佐藤, 充

• 木谷, 裕

• 上西, 博英

抄録

The NLRC4 inflammasome, which recognizes flagellin and components of the type III secretion system, plays an important role in the clearance of intracellular bacteria. Here, we examined the genomic sequences carrying two genes encoding key components of the NLRC4 inflammasome—NLR family, CARD-containing 4 (NLRC4), and NLR apoptosis inhibitory protein (NAIP)—in pigs. Pigs have a single locus encoding NLRC4 and NAIP. Comparison of the sequences thus obtained with the corresponding regions in humans revealed the deletion of intermediate exons in both pig genes. In addition, the genomic sequences of both pig genes lacked valid open reading frames encoding functional NLRC4 or NAIP protein. Additional pigs representing multiple breeds and wild boars also lacked the exons that we failed to find through genome sequencing. Furthermore, neither the NLRC4 nor the NAIP gene was expressed in pigs. These findings indicate that pigs lack the NLRC4 inflammasome, an important factor involved in monitoring bacterial proteins and contributing to the clearance of intracellular pathogens. These results also suggest that genetic polymorphisms affecting the molecular functions of TLR2, TLR4, TLR5, and other pattern recognition receptors associated with the recognition of bacteria have a more profound influence on disease resistance in pigs than in other species.

source:SC30201802140027

source:NARO成果DBa

source:This is a post-peer-review, pre-copyedit version of an article published in [Immunogenetics].

source:The final authenticated version is available online at: http://dx.doi.org/10.1007/s00251-016-0955-5

source:This is not the published version. Please cite only the published version.

source:この論文は出版社版ではありません。引用の際には出版社版をご利用ください。

source:https://dx.doi.org/10.1007/s00251-016-0955-5

収録刊行物

• Immunogenetics = Immunogenetics

  Immunogenetics = Immunogenetics 69 (2), 125-130, 2017-02

  Published by Springer Berlin Heidelberg

キーワード

• Genome sequencing
• Inflammasome
• Pattern recognition receptors
• Swine

詳細情報

• CRID: 1050282814246326784
• NII論文ID: 120006776865
• ISSN: 14321211
• Web Site: http://id.nii.ac.jp/1578/00003212/
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles