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- 草野, 博彰
- Research Institute for Sustainable Humanosphere, Kyoto University
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- 矢崎, 一史
- Laboratory of Medicinal Cell Biology, Kobe Pharmaceutical University
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- 杉山, 暁史
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Ogita, Shinjiro
- Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima
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- Yazaki, Kazufumi
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Sugiyama, Akifumi
- Research Institute for Sustainable Humanosphere, Kyoto University
書誌事項
- タイトル別名
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- Uptake of adenine by purine permeases of Coffea canephora
抄録
Purine permeases (PUPs) mediate the proton-coupled uptake of nucleotide bases and their derivatives into cytosol. PUPs facilitate uptake of adenine, cytokinins and nicotine. Caffeine, a purine alkaloid derived from xanthosine, occurs in only a few eudicot species, including coffee, cacao, and tea. Although caffeine is not an endogenous metabolite in Arabidopsis and rice, AtPUP1 and OsPUP7 were suggested to transport caffeine. In this study, we identified 15 PUPs in the genome of Coffea canephora. Direct uptake measurements in yeast demonstrated that CcPUP1 and CcPUP5 facilitate adenine – but not caffeine – transport. Adenine uptake was pH-dependent, with increased activity at pH 3 and 4, and inhibited by nigericin, a potassium–proton ionophore, suggesting that CcPUP1 and CcPUP5 function as proton-symporters. Furthermore, adenine uptake was not competitively inhibited by an excess amount of caffeine, which implies that PUPs of C. canephora have evolved to become caffeine-insensitive to promote efficient uptake of adenine into cytosol.
収録刊行物
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- Bioscience, biotechnology, and biochemistry
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Bioscience, biotechnology, and biochemistry 83 (7), 1300-1305, 2019
Informa UK Limited
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詳細情報 詳細情報について
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- CRID
- 1050002213761137280
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- NII論文ID
- 120006796496
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- ISSN
- 09168451
- 13476947
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- HANDLE
- 2433/245789
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
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