High hydrostatic pressure induces vigorous flagellar beating in Chlamydomonas non-motile mutants lacking the central apparatus

HANDLE オープンアクセス
  • 西山, 雅祥
    Department of Life Sciences, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima
  • Nishiyama, Masayoshi
    The Hakubi Center for Advanced Research, Kyoto University・Department of Physics, Faculty of Science and Engineering, Kindai University

抄録

The beating of eukaryotic flagella (also called cilia) depends on the sliding movements between microtubules powered by dynein. In cilia/flagella of most organisms, microtubule sliding is regulated by the internal structure of cilia comprising the central pair of microtubules (CP) and radial spokes (RS). Chlamydomonas paralyzed-flagella (pf) mutants lacking CP or RS are non-motile under physiological conditions. Here, we show that high hydrostatic pressure induces vigorous flagellar beating in pf mutants. The beating pattern at 40 MPa was similar to that of wild type at atmospheric pressure. In addition, at 80 MPa, flagella underwent an asymmetric-to-symmetric waveform conversion, similar to the one triggered by an increase in intra-flagella Ca²⁺ concentration during cell’s response to strong light. Thus, our study establishes that neither beating nor waveform conversion of cilia/flagella requires the presence of CP/RS in the axoneme.

収録刊行物

キーワード

詳細情報 詳細情報について

  • CRID
    1050283688738067456
  • NII論文ID
    120006799256
  • ISSN
    20452322
  • HANDLE
    2433/245810
  • 本文言語コード
    en
  • 資料種別
    journal article
  • データソース種別
    • IRDB
    • CiNii Articles

問題の指摘

ページトップへ