擬似微小重力及び過重力下における骨代謝制御:培養ウロコを用いた解析

• 鈴木, 信雄

• 大森, 克徳

• 井尻, 憲一

• 北村, 敬一郎

• 清水, 宣明

• 田畑, 純

• 池亀, 美華

• 中村, 正久

• 近藤, 隆

• 松田, 恒平

• Suzuki, Nobuo

• Omori, Katsunori

• Ijiri, Kenichi

• Kitamura, Keiichiro

• Shimizu, Nobuaki

• Tabata, Makoto J.

• Ikegame, Mika

• Nakamura, Masahisa

• Kondo, Takashi

• Matsuda, Kohei

書誌事項

タイトル別名

• Regulation of bone metabolism under simulated micro-gravity and hyper-gravity loading: Analysis by the cultured scales of goldfish

抄録

Fish scale is a calcified tissue that contains osteoblasts, osteoclasts, and bone matrix, all of which are similar to those found in mammalian bone. Recently, we developed a new in vitro model system using goldfish scale. This system can detect the activities of osteoblasts and osteoclasts with AlkaLine Phosphatase (ALP) and Tartrate-Resistant Acid Phosphatase (TRAP) as the respective markers and analyze the co-relationship between osteoblasts and osteoclasts. Using this system, we indicated that osteoblastic and osteoclastic activities in the scale responded to the simulated microgravity with a three-dimensional clinostat. As these activities in the scale did not change by the stimulation with a two-dimensional clinostat, we believe that the response to simulated microgravity in the scale osteoblasts and osteoclasts is a specific phenomenon. In addition, we analyzed the bone metabolism under 2-, 4-, and 7-gravity (G) loading with a centrifuge and compared them with the control (1-G). The osteoblastic activity significantly increased under 2- and 4-G loading. This activity increased remarkably under 7-G loading. On the other hand, we found that the osteoclastic activity significantly decreased under 2-G loading. Under 4-G loading, there was no significant difference between G-loaded scales and control scales. The osteoclastic activity tended to increase under 7-G loading. These results were similar to our previous study of acceleration by vibration. With the goal of developing a drug for bone diseases, the effect of novel bromomelatonin derivatives on osteoblasts and osteoclasts was examined. All bromomelatonin derivatives had an inhibitory action on osteoclasts. In particular, 1-benzyl-2, 4, 6-tribromomelatonin (benzyl-tribromomelatonin) possessed a stronger activity than melatonin. In reference to osteoblasts, all bromomelatonin derivatives had a stimulatory action. In addition, estrogen receptor mRNA (messenger Ribonucleic Acid) expression (an osteoblastic marker) was increased in benzyl-tribromomelatonin (10(exp -7) M)-treated scales. Therefore, this chemical will be useful to treat bone diseases, such as those experienced in space flight.

資料番号: AA0063706059

収録刊行物

• 宇宙利用シンポジウム　第24回　平成19年度 = Space Utilization Research: Proceedings of the Twenty-fourth Space Utilization Symposium

  宇宙利用シンポジウム　第24回　平成19年度 = Space Utilization Research: Proceedings of the Twenty-fourth Space Utilization Symposium 230-233, 2008-03

  宇宙航空研究開発機構宇宙科学研究本部

キーワード

• 骨芽細胞
• 破骨細胞
• 魚鱗
• 魚
• 代謝
• 細胞培養
• クリノスタット
• 微小重力
• 遠心機
• 高重力環境
• ブロモメラトニン
• 金魚
• 重力効果
• 活性
• osteoblast
• osteoclast
• fish scale
• fish
• metabolism
• cell culturing
• clinostat
• microgravity
• centrifuge
• high gravity environment
• bromomelatonin
• Carassius auratus
• gravitational effect
• activity

詳細情報

• CRID: 1050574036224540672
• NII論文ID: 120006830679
• Web Site: http://id.nii.ac.jp/1696/00013429/
• 本文言語コード: ja
• 資料種別: conference paper
• データソース種別:
  • IRDB
  • CiNii Articles
  • KAKEN