Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon)

DOI HANDLE Web Site 10 Citations 25 References Open Access

Abstract

The Bonin flying fox (Pteropus pselaphon) is one of the most critically endangered species of animals. The number of this species is estimated to be around 150; being classified at the top rank in the list by International Union of Animal Conservation. Our group previously showed that expression of CDK4, CYCLIN D1, and telomerase reverse transcriptase (TERT) efficiently induce immortalization of human, bovine, swine, monkey, and buffalo-derived cells. In this manuscript, we successfully established the primary cells from Bonin flying fox. We introduced CDK4, CYCLIN D1, and TERT into the primary cells. The established cells showed efficient expression of introduced genes at the protein level. Furthermore, the established cells were free from senescence, indicating it reached to immortalization. Moreover, we showed that interspecies somatic cell nuclear transfer of Bonin flying fox derived cell into bovine embryo allowed the development of the embryo to 8 cell stages. Our established cell has the potential to contribute to species conservation.

Correction -- 29 May 2020: Tani T, Eitsuka T, Katayama M, Nagamine T, Nakaya Y, et al. (2020) Correction: Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon). PLOS ONE 15(5): e0234054.

Journal

  • PLOS ONE

    PLOS ONE 14 (8), e0221364-, 2019-08-26

    Public Library of Science (PLoS)

Citations (10)*help

See more

References(25)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top