A novel sponge-derived protein thrombocorticin is a new agonist for thrombopoietin receptor

• Watari, Hiromi

• Nakajima, Hiroya

• Atsuumi, Wataru

• Nakamura, Takanori

• Nanya, Takeshi

• Ise, Yuji

• Sakai, Ryuichi

抄録

We screened 868 marine extracts in search of hematopoietic molecules resulted in findings of several extracts that proliferated Ba/F3-HuMpl cells but not the cells expressed with other hematopoietic cytokine receptors, EPO and G-CSF. Separation of the most potent extract of a Micronesian sponge Corticium sp., guided by the cell proliferation assay using Ba/F3-HuMpl cells resulted in an isolation of thrombocorticin (ThC), a novel 14 kDa protein as an active principal. ThC displayed concentration-dependent proliferation of Ba/F3-HuMpl cells, and had a stronger activity than that of eltrombopag, a small molecule drug used to treat thrombocytopenia. ThC induced phosphorylation of STAT5, suggesting that it activates Jak/STAT pathway as in the case of TPO. These results together indicated that ThC is a specific agonist for c-Mpl, although the size and shape differs largely from TPO. Here we present isolation, characterization and biological activity of ThC.

収録刊行物

• Comparative biochemistry and physiology Part C : Toxicology & pharmacology

  Comparative biochemistry and physiology Part C : Toxicology & pharmacology 221 82-88, 2019-07

  Elsevier

キーワード

• Hematopoietic cytokine
• Thrombopoietin
• Agonist
• Marine sponge
• Protein

詳細情報

• CRID: 1050848249755100288
• NII論文ID: 120006864488
• NII書誌ID: AA11504679
• DOI: 10.1016/j.cbpc.2019.04.003
• HANDLE: 2115/78805
• ISSN: 15320456
• Web Site: https://api.elsevier.com/content/article/PII:S153204561930081X?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S153204561930081X?httpAccept=text/plain
• 本文言語コード: en
• 資料種別: journal article
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