Mesenchymal stromal cells in bone marrow express adiponectin and are efficiently targeted by an adiponectin promoter-driven Cre transgene

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  • 原, 崇裕
    Laboratory of Immune Regulation, Department of Virus Research, Institute of Frontier Life and Medical Sciences, Kyoto University･Graduate School of Medicine, Kyoto University
  • 阿部, 真也
    Laboratory of Immune Regulation, Department of Virus Research, Institute of Frontier Life and Medical Sciences, Kyoto University
  • 谷一, 靖江
    Laboratory of Immune Regulation, Department of Virus Research, Institute of Frontier Life and Medical Sciences, Kyoto University･Graduate School of Medicine, Kyoto University
  • 生田, 宏一
    Graduate School of Medicine, Kyoto University･Graduate School of Medicine, Kyoto University
  • Sehara-Fujisawa, Atsuko
    Laboratory of Tissue Stem Cell Biology, Department of Regeneration Science and Engineering, Institute of Frontier Life and Medical Sciences, Kyoto University
  • Nagasawa, Takashi
    Laboratory of Stem Cell Biology and Developmental Immunology, Graduate School of Frontier Biosciences and Graduate School of Medicine, Osaka University
  • Tobe, Kazuyuki
    First Department of Internal Medicine, Faculty of Medicine, University of Toyama
  • Ikuta, Koichi
    Laboratory of Immune Regulation, Department of Virus Research, Institute of Frontier Life and Medical Sciences, Kyoto University

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Stromal cells in bone marrow (BM) constitute a specific microenvironment supporting the development and maintenance of hematopoietic cells. Adiponectin is a cytokine secreted by adipocytes. Besides its anti-diabetic and anti-atherogenic roles, adiponectin reportedly regulates the development and function of hematopoietic cells in BM. However, it remains unclear whether mesenchymal stromal cells in BM express adiponectin. Here, we show that PDGFRβ+VCAM-1+ stromal cells express adiponectin. Lineage tracing revealed that a majority of PDGFRβ+VCAM-1+ cells were targeted by an adiponectin promoter-driven Cre (Adipoq-Cre) transgene. Additionally, the Adipoq-Cre transgene targets a minority of osteoblasts at a younger age but larger populations are targeted at an older age. Furthermore, the Adipoq-Cre transgene targets almost all CXCL12-abundant reticular (CAR) cells and most of the stromal cells targeted by the Adipoq-Cre transgene are CAR cells. Finally, deletion of interleukin-7 (IL-7) by the Adipoq-Cre transgene resulted in severe impairment of B lymphopoiesis in BM. These results demonstrate that PDGFRβ+VCAM-1+ stromal cells in BM express adiponectin and are targeted by the Adipoq-Cre transgene, suggesting a broader specificity of the Adipoq-Cre transgene.

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