Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

• Hashimoto, Masakazu

• Takemoto, Tatsuya

Abstract

Recent use of the CRISPR/Cas9 system has dramatically reduced the time required to produce mutant mice, but the involvement of a time-consuming microinjection step still hampers its application for high-throughput genetic analysis. Here we developed a simple, highly efficient and large-scale genome editing method, in which the RNAs for the CRISPR/Cas9 system are electroporated into zygotes rather than microinjected. We used this method to perform single-stranded oligodeoxynucleotide (ssODN)-mediated knock-in in mouse embryos. This method facilitates large-scale genetic analysis in the mouse.

Journal

• Scientific Reports

  Scientific Reports 5 11315-, 2015-06-11

  Springer Nature

Details

• CRID: 1050848249856228864
• NII Article ID: 120006875680
• ISSN: 20452322
• Web Site: http://repo.lib.tokushima-u.ac.jp/114972
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles