Novel Fluorescence-Based Method To Characterize the Antioxidative Effects of Food Metabolites on Lipid Droplets in Cultured Hepatocytes

• Tsukui, Takayuki

• Chen, Zhen

• Fuda, Hirotoshi

• Furukawa, Takayuki

• Oura, Kotaro

• Sakurai, Toshihiro

• Hui, Shu-Ping

• Chiba, Hitoshi

抄録

A fluorescence microscopic method for characterizing size, quantity, and oxidation of lipid droplets (LDs) in HepG2 cells was developed. LDs were induced by palmitic (PA), oleic (OA), or linoleic acids (LA) and stained with two fluorescent probes for neutral lipids and lipid peroxides. Each fatty acid increased the number of LDs and oxidized LDs (oxLDs) and the degree of LD oxidation time dependently, as well as increased intracellular triglyceride hydroperoxides. LDs induced by LA without 2,2′-azobis(2-amidinopropane)dihydrochloride (AAPH) showed the most significant oxidation degree over PA and OA, especially in large LDs (area ≥ 3 μm2, oxLD/LD = 52.3 ± 21.7%). Under this condition, two food-derived antioxidants were evaluated, and both of them significantly improved the LD characteristics. Moreover, chlorogenic acid reduced the quantity of large LDs by 74.0–87.6% in a dose-dependent manner. The proposed method provides a new approach to evaluate the effect of dietary antioxidants on LD characteristics.

収録刊行物

• Journal of Agricultural and Food Chemistry

  Journal of Agricultural and Food Chemistry 67 (35), 9934-9941, 2019-08-11

  ACS

キーワード

• lipid droplet (LD)
• fluorescence microscopy
• antioxidant
• lipid oxidation
• non-alcoholic fatty liver disease (NAFLD)

詳細情報

• CRID: 1050566774891092352
• NII論文ID: 120006877261
• NII書誌ID: AA00250794
• DOI: 10.1021/acs.jafc.9b02081
• ISSN: 15205118
• ISSN: 00218561
• HANDLE: 2115/79057
• Web Site: https://pubs.acs.org/doi/pdf/10.1021/acs.jafc.9b02081
• 本文言語コード: en
• 資料種別: journal article
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