The Protective Effect of Chlorogenic Acid on Vascular Senescence via the Nrf2/HO-1 Pathway

• Hada, Yoshiko

• Uchida, Haruhito A.

• Otaka, Nozomu

• Onishi, Yasuhiro

• Okamoto, Shugo

• Nishiwaki, Mariko

• Takemoto, Rika

• Takeuchi, Hidemi

• Wada, Jun

抄録

The world faces the serious problem of aging. In this study, we aimed to investigate the effect of chlorogenic acid (CGA) on vascular senescence. C57/BL6 female mice that were 14 +/- 3 months old were infused with either Angiotensin II (AngII) or saline subcutaneously for two weeks. These mice were administered CGA of 20 or 40 mg/kg/day, or saline via oral gavage. AngII infusion developed vascular senescence, which was confirmed by senescence associated-beta-galactosidase (SA-beta-gal) staining. CGA administration attenuated vascular senescence in a dose-dependent manner, in association with the increase of Sirtuin 1 (Sirt1) and endothelial nitric oxide synthase (eNOS), and with the decrease of p-Akt, PAI-1, p53, and p21. In an in vitro study, with or without pre-treatment of CGA, Human Umbilical Vein Endothelial Cells (HUVECs) were stimulated with H(2)O(2)for an hour, then cultured in the absence or presence of 0.5-5.0 mu M CGA for the indicated time. Endothelial cell senescence was induced by H2O2, which was attenuated by CGA treatment. Pre-treatment of CGA increased Nrf2 in HUVECs. After H(2)O(2)treatment, translocation of Nrf2 into the nucleus and the subsequent increase of Heme Oxygenase-1 (HO-1) were observed earlier in CGA-treated cells. Furthermore, the HO-1 inhibitor canceled the beneficial effect of CGA on vascular senescence in mice. In conclusion, CGA exerts a beneficial effect on vascular senescence, which is at least partly dependent on the Nuclear factor erythroid 2-factor 2 (Nrf2)/HO-1 pathway.

収録刊行物

• International Journal of Molecular Sciences

  International Journal of Molecular Sciences 21 (12), 4527-, 2020-06-25

  MDPI

キーワード

• chlorogenic acid
• vascular senescence
• nuclear factor erythroid 2-related factor 2
• heme oxygenase-1

詳細情報

• CRID: 1050566774908629376
• NII論文ID: 120006877909
• ISSN: 16616596
• ISSN: 14220067
• DOI: 10.3390/ijms21124527
• Web Site: https://ousar.lib.okayama-u.ac.jp/60284
• Web Site: https://www.mdpi.com/1422-0067/21/12/4527/pdf
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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