Glycosylinositol phosphoceramide-specific phospholipase D activity catalyzes transphosphatidylation

• Hasi, Rumana Yesmin

• Miyagi, Makoto

• Morito, Katsuya

• Ishikawa, Toshiki

• Kawai-Yamada, Maki

• Imai, Hiroyuki

• Fukuta, Tatsuya

• Kogure, Kentaro

• Kanemaru, Kaori

• Hayashi, Junji

• Kawakami, Ryushi

• Tanaka, Tamotsu

Bibliographic Information

Other Title

• Transphosphatidylation by GIPC-PLD

Abstract

Glycosylinositol phosphoceramide (GIPC) is the most abundant sphingolipid in plants and fungi. Recently, we detected GIPC-specific phospholipase D (GIPC-PLD) activity in plants. Here, we found that GIPC-PLD activity in young cabbage leaves catalyzes transphosphatidylation. The available alcohol for this reaction is a primary alcohol with a chain length below C4. Neither secondary alcohol, tertiary alcohol, choline, serine nor glycerol serves as an acceptor for transphosphatidylation of GIPC-PLD. We also found that cabbage GIPC-PLD prefers GIPC containing two sugars. Neither inositol phosphoceramide, mannosylinositol phosphoceramide nor GIPC with three sugar chains served as substrate. GIPC-PLD will become a useful catalyst for modification of polar head group of sphingophospholipid.

Journal

• The Journal of Biochemistry

  The Journal of Biochemistry 166 (5), 441-448, 2019-08-26

  Oxford University Press

Keywords

• Sphingolipid
• Phytoceramide 1-phosphate
• Glycosylinositol phosphoceramide
• Phospholipase D

Details

• CRID: 1050285299951137024
• NII Article ID: 120006879927
• NII Book ID: AA12096002
• ISSN: 17562651
• ISSN: 0021924X
• DOI: 10.1093/jb/mvz056
• NDL BIB ID: 030083624
• Web Site: http://repo.lib.tokushima-u.ac.jp/113685
• Web Site: http://id.ndl.go.jp/bib/030083624
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030083624
• Web Site: http://academic.oup.com/jb/advance-article-pdf/doi/10.1093/jb/mvz056/30010946/mvz056.pdf
• Web Site: http://academic.oup.com/jb/article-pdf/166/5/441/30259884/mvz056.pdf
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN